ABSTRACT
Female sex pheromone production in certain moth species have been shown to be regulated by a cephalic endocrine peptidic factor: pheromone biosynthesis activating neuropeptide (PBAN), having 33 amino acid residues. Antisera against synthetic Heliothis zea-PBAN were developed. Using these polyclonals, immunoreactivity was mapped in the nervous system of Achaea janata. Three distinct groups of immunopositive secretory neurons were identified in the suboesophageal ganglion; and immunoreactivity was observed in the corpora cardiaca, thoracic and in the abdominal ganglia. From about 6000 brain sub-oesophageal ganglion complexes, the neuropeptide was isolated; and purified sequentially by Sep-pak and reversed phase high performance liquid chromatographic methods. Identity of purified PBAN fraction was confirmed with polyclonal antibody by immunoblotting. Molecular mass of the isolated peptide was determined by matrix-assisted laser desorption/ionization mass spectrometry, and was found to be 3900 Da, same as that of known H. zea-PBAN. Radiochemical bioassay confirmed the pheromonotropic effect of the isolated neuropeptide in this insect.
Subject(s)
Animals , Brain/metabolism , Chromatography, High Pressure Liquid , Ganglia, Invertebrate/metabolism , Immunoblotting , Immunohistochemistry , Larva/metabolism , Moths/metabolism , Neurons/metabolism , Neuropeptides/isolation & purificationABSTRACT
A nivel de Sistema Nervioso Central coexisten múltiples neurotransmisores y neuromoduladores que a través de complejas interrelaciones participan en la regulación del flujo y perfusión cerebral tanto en condiciones normales como patológicas. A continuación se revisarán los principales neuropéptidos vasculares reconocidos hasta el momento, que participan en esta regulación